Phylogenetic characterization of some Bovine viral diarrhea viruses in Egypt
Abstract: Sixty seven byffy coat specimens, obtained from clinically suspected cattle at farms located in Belbees. El-sharquia, were screened for BVDV. Only9/67 of the tested specimens (13%) were BVDV positive by reverse transcription-polymerase chain reaction (RT-PCR). Whereas, virus isolation followed by immunostaining procedure in cell culutre confirmed only 6/9 BVDVs that were all cytopathogenic. Direct sequencing of the RT-PCR amplicons revealed an extreme nucleotide sequence homology (>98%) among all tested Egyptian isolates versus the local vaccinal strain. Sequence alignments showed variable identitiy (74%-93%) of the Egyptian vaccinal strain versus refernce laboratory and vaccinal strains of BVDV. Clustring and phylogenetic analyses suggested that these Egyptian vaccinal and field BVDVs are low-Kinetic varaints of refernce BVDV-la. particulary the NADL. Represented as a distinguished branch within the phylogenetic tree. Accordingly, BVDV-la (NADL -like). is the domininat circulating genotype in Belbees. El-shraquia, to date, and the local vaccine could likely induce an antigenically appropiate immune response. The present study offers a useful molecular epizootiological tool to group local BVDVs in a herd-specific manner at a vaccines and diagnostic assays for effective control policies.
Publication year 2004
Pages 421-435
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City Cairo
serial title The Egyptian journal of virology
Author(s) from ARC
External authors (outside ARC)
    S.vilcek Department of infectious& Tropical diseases. University of veterinary medicine Slovack republic
Agris Categories Animal diseases
Proposed Agrovoc Bovine viral diarrhea;
Publication Type Journal
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