Preparation of Diagnostic LYSATE Antigens for rapid evaluation of local ENTERO-3 Vaccine

Abstract: Studies were conducted to determine the utility of lysate antigens for rapid evaluation of the local entero-3 vaccine, antigens were prepared from cell cultures infected with bovine rota virus (BRV) and bovine corona virus (BCV) as well as from Enterotoxigenic E.coli strain K99. Prepared antigens were tested with field serum samples collected from both late pregnant entero-3 vaccinated cows and their offsprings using different serological assays including : microagglutination test, indirect ELISA and immunofluorescent antibody technique. Results of their endeavour were correlated to that of the standard virus neutralization test. The locally prepared antigens were proved useful for vaccine evaluation. Moreover, these antigens are recommended for both detection and assessment post vaccination or post infection of sero-conversion against BRV, BCV and E.Coli.
Publication year 2005
Pages 181-189
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City Beni-Suef
serial title Future of animal wealth in Egypt under GATT agreement
Author(s) from ARC
External authors (outside ARC)
    Amal M El-Sawah Central laboratory for quality control of veterinary biologics
    Mohamed Saed Wassel
Agris Categories Animal diseases
AGROVOC
TERMS
Antigens. Vaccines.
Publication Type Conference/Workshop