Evaluation of locally produced serums as nutritive supplements for cell culture media

Abstract: 7 serum batches viz., cross-breed Friesian cattle, adult buffalo, Balady cattle, adult camel, imported American, and imported Austrian cattle serums as well as newly born buffalo calf serums were investigated as cell culture supplements in our herein studies.
The first three serum batches exhibited the highest total protein, Albumin and globulin values being 9.39 / 3.08/ 6.31 ; 8.21/ 3.18/ 5.03 ; 8.23/2.7/ 5.53 g % respectively. Moderate estimates were observed in the second three serum batches which were 6.67 / 1.58 / 5.09 ; 5.97 / 2.42 /3.55 and 5.88 / 2.1/ 3.78g % respectively. The least values were found in the seventh serum batch being 2.97 / 1.25 / 1.72g %. The growth promoting pontentialities on vero cells for the forementioned serum batches in terms of cell counts were of 8.3, 8.6, 8.2, 6.7, 6.66, 6.4 and 5.2 X 106 cell/ml respectively. The total protein, albumin and globulin ratios of the first three serum batches were reestimated after their treatment with 10% polyethylene Glycol (PEG), being 7.29 / 2.78 / 4.51; 6.32 /2.97/ 3.35 ; 6.2 / 2.4/3.8 g % respectively. The growth promoting characteristics of the three treated serum batches on vero cells revealed a mild reduction in cell counts being 6.9, 7.1 and 6.7X106/ml respectively. It has been found that the serum obtained from whole blood with ordinary clotthing system enjoyed higher protein fractions than those treated with 10% PEG (including the two imported serum batches) as they possessed the Platelet Derived Growth Factor (PDGF), while the treatment of serum batches with PEG has led to considerable decrease in protein fractions especially globulins but it did not dramatically affect the growth promoting affinities of the treated batches. Our speculations drew the attention to the efficacy of utilizing of Sera of buffalo and Camels as supplements for cell cultures as they gave growth capabilities like those of bovine origns. In addition to their being nonexpensive as supplements for cell cultures that consequently reflects on the industry of vaccines and specific antisera.
Publication year 1999
Pages 1130-1139
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City cairo
serial title 24th arab veterinary medical congress
Author(s) from ARC
Agris Categories Agriculture - General aspects
AGROVOC
TERMS		 Cell culture. Evaluation. Nutritive value.
Publication Type Journal
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