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Conjugation of a PPR Hyper Immune Serum with Fluorescine Isothiocyanate for Serological uses
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Abstract: In the present study; a PPR hyper immune serum was prepared in local breed of goats. It was found to have specific PPR neutralizing antibodies of a titer 1024; total protein, albumin and globulin ratios of 5.9±0.21, 2.87±0.18 and 3.07±11gm% respectively. The immune globulin in such serum was precipitated using ammonium sulphate, purified and conjugated with flouresine isothiocyanate. This prepared hyper immune serum was evaluated by applying the fluorescent antibody technique on VERO cells cultured slides infected with PPR virus using different dilutions of the conjugated serum. Positive fluorescent reaction was obtained up to a conjugate dilution of 1:10000 as the results obtained on the use of imported conjugate. So, it could be concluded that the locally prepared PPR hyper immune serum conjugated with fluorescine isothiocyanate could be used in serological tests and have the same value of the imported one which is not usually available and of high cost.
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| Publication year |
2002
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| Pages |
307-314
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| Availability location |
معهد بحوث الامصال واللقاحات البيطرية
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| Availability number |
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| Organization Name |
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| City |
Minufiya
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| serial title |
The second veterinary congress
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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| Proposed Agrovoc |
المجترات -أمراض;
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| Publication Type |
Conference/Workshop
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