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Assessment of a Probable Stabilizing Potentiative Effect of Trehalose Incorporation through PPR Attenuated Vaccine Production
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Abstract: An introduced technical modification through the manufacture of live PPR vaccine was the incorporation of 1% trehalose dihydrate with the conventional 2.5% lactalbumin hydrolysate 5%-sucrose-stabilizer, just prior to lyophilization. This modification was proven quite efficacious to potentiate the thermotolerance of the lyophilized trehalose-treated PPR live vaccine as evidenced by minimized loss in virus TCID50 titers post exposure of the dried vaccine to 37oC as well as to 45oC for certain intervals. Such a modification did not interfere with the integrity of the vaccine virus particles as evidenced by significant neutralizing antibody response provoked in sheep inoculated with the trehalose-treated vaccine even though exposed to such temperatures.
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| Publication year |
2008
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| Pages |
513-521
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| Availability location |
معهد بحوث الامصال واللقاحات البيطرية
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| Availability number |
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| Organization Name |
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| City |
المنوفية
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| serial title |
veterinary medicine and animal wealth development
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| Author(s) from ARC |
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| Agris Categories |
Animal diseasesMiscellaneous animal disordersVeterinary science and hygiene - General aspects
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AGROVOC
TERMS |
Incorporation.
Pest of small ruminants.
Trehalose.
Vaccines.
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| Publication Type |
Journal
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