The use of trehalose in the preparation and preservation of rinderpest and peste des petits ruminants vaccines
Abstract: The present study was aiming for the production and evaluation of thermo tolerated lyophilized rinderpeste and (OIE,2004). Peste des petits ruminants (PPR) live vaccines ; through the incorporation of the disaccharide trehalose dehydrate.
Firstly this sugar was proven as a safe material to Vero cells ; the substrate for manufacturing both vaccines. Vero cell non-toxicity was sustainable with this sugar throughout a dosing range of 0.5 and 6.0 mg/ml of either growth or maintenance media. The technical modification was the incorporation of 1.0 mg of this sugar per ml of maintenance media overlaying virus inoculated Vero cells, coupled with mixing the virus harvest with an equal volume of an aqueous solution of 5 % trehalose dehydrate as a cryoprotectant exicipient, through manufacturing both rinderpest and PPR live vaccines ; being subjected to lyophilization, thereafter.
This modification was proven to be the sole attribute of the highly exalted thermotolerated potency of both vaccines ; post lyophilization exposure to either 37°C or 45°C for two weeks, as evidenced by virus titration tests. Required number of TCID50 log5 of virus per dose per animal were found to be achieved for both vaccines under these circumstances.
The modified vaccines were proven safe and potent in susceptible host animals through recommended tests that were equally undertaken with conventionally manufactured rinderpest and PPR vaccines.
Such an achievement would be having a remarkable interest when launching rinderpest and PPR vaccination campaigns in hot climate districts lacking the cold chain facility.
Publication year 2005
Pages 1343-1360
Availability location معهد بحوث الامصال واللقاحات البيطرية
Availability number
Organization Name
City المنصورة
serial title المؤتمر الدولي العلمي الرابع تحت عنوان
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Vaccines.
Proposed Agrovoc Ruminants vaccines;
Publication Type Conference/Workshop
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