Preparation and evaluation of lumpy skin disease hyperimmune serum conjugated with florescein
Abstract: Hyper immune serum against lumpy skin disease virus (LSDV) was prepared by inoculation of the virus in successive doses into susceptible two calves with complete and incomplete freund's adjuvant. Identity tests were applied by agar gel precipitation test (AGPT) and indirect fluorescent antibody technique (IFAT). Serum neutralization test (SNT) and ELISA tests were applied for titration and evaluation of the hyper immune sera before conjugation with fluorescent. Total protein CONCENTRATION OF THE PREPARED LSDV ANTISERA WAS 0.89 G/DL . Separation of anti-LSDV immunoglobulins IgG were done using ammonium sulphate followed by conjugation with flurescein isothiocyanate at pH 9.6 . The anti-LSDV IgG conjugated flurescein sterile and was used to detect LSDV in the MDBK cells and gave good results to dilution 1/20 while the references conjugate to 1/30.
Publication year 2008
Pages 4-5
Availability location معهد بحوث الامصال واللقاحات البيطرية
Availability number
Organization Name
City القاهرة
serial title The Egyptain journal of virology
ISSN 1685-1687
Author(s) from ARC
External authors (outside ARC)
    ماجدة محمد سيد المعمل المركزي للرقابة على المستحضرات البيطرية
Agris Categories Animal diseases
AGROVOC
TERMS		 Capripoxvirus. Immune serum. Viroses.
Proposed Agrovoc florescein;
Publication Type Journal
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