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Preliminary study for preparation of serological and diagnostic conjugated fluorescent hyper immune serum against fowl spirochaetosis.
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Abstract: A specific hyperimmune serum was prepared in chickens and rabbit and precipitated with ammonium sulphate. The obtained immunoglobulins lgG were conjugated with fluorescein isothiocyanate for performing of fluorescent antibody technique ( FAT) for serological diagnosis of avian spirochaetosis in - vitro. It was found that using of methyl alcohol (methanol) in fixation of berrelia anserine was better than using acetone. The conjugated hyperimmune serum prepared in chicken gave positive reaction up to dilution 1:1024 in the direct technique in comparison with that prepared in rabbits which gave positive reaction up to 1:512 dilution. Results of the indirect technique were better than that obtained by using direct technique due to absence of the non-specific reaction in the indirect technique. Non –infected samples showed a black back ground in negative reaction while positive reaction provides the specific yellow green (apple green) light under fluorescent microscope.
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| Publication year |
2007
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| Pages |
165-173
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| Availability location |
مكتبة معهد بحوث الامصال واللقاحات البيطرية.
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| Availability number |
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| Organization Name |
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| serial title |
مجلة الجمعية الطبية البيطرية المصرية
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| Author(s) from ARC |
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AGROVOC
TERMS |
Vaccines.
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| Proposed Agrovoc |
Chickens diseases control;Poultry Vaccination;Immune serums.;Rabbits Diseases;Spirochaetosis;
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| Publication Type |
Journal
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