Investigation of the effect of different adjuvants in the inactivated canine parvo vaccine on the immune response of puppies
Abstract: Four experimental batches of inactivated canine parvo vaccine were prepared using three different oils adjuvant ; Ressela and Montonid (ISA-50) as mineral oils and peanut oil as vegetable oil and aluminum hydroxide gel adjuvant. These vaccine batches were evaluated in a comparison with the live attenuated CPV vaccine. Each one of the four vaccine formula was inoculated I/M in a group of puppies (3-4 months of age) and S/C in another puppy group. Unvaccinated puppy group was kept as test control. Serum samples were obtained from all experimental animals weekly up to 8 weeks post vaccination to monitor the levels of induced canine parvo antibodies. Serum neutralization test (SNT): haemagglutination inhibition test (HI) and Enzyme linked immuno sorbent assay (ELIZA) revealed that Montanid oil induced the highest levels of immune response followed by that induced by resella and peanut oil and lastly that induced by aluminum hydroxide gel. Also it was noticed that there was no noticeable difference between the levels of antibodies induced by either route of inoculation post vaccination and challenge. The result of challenge test of vaccinated and unvaccinated revealed that all vaccinated animals were able to withstand the virus but in difference degrees agree with the level of the present antibodies while unvaccinated puppies showed typical symptoms of canine parvo and death
Publication year 2007
Pages 172-178
Availability location معهد بحوث الأمصال واللقاحات البيطرية
Availability number
Organization Name
City Zagazig
serial title Zagazig veterinary Journal
ISSN 1110-1458
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Canine parvovirus. Immune response. Puppies. Vaccines.
Publication Type Journal
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