The effect of dimethyl dioctadecyl ammonium bromide (DDA) with BCG in laboratory animals experimentally infected with salmonella typhimurium
Abstract: Immunoadjuvant effect of Dimethyl ammonium (DDA) was evaluated on laboratory animals immunized with BCG. Guinea pigs and mice were classified in to 7groups, the first groups were immunized with living attenuated BCG (BCG-LA), BCG culture filtrate (BCG-CF), heat killed BCG (BCG-HK) and (DDA) respectively. The 5th group was immunized with (DDA+BCG-HK) while the 6th group was immunized with (DDA+BCG-CF) respectively. The last group was left as control non immunized animals. All immunized laboratory animals developed clear cell mediated immunity rather than control groups. The cell mediate immune response is more pronounced in immunized mice than guinea pigs. The humeral antibody response in guinea pig recoded higher (1/160) in groups (1 and 5), while in mice groups, the level of antibody was observed higher (1/320) in group (5) and noted (1/160) in groups (1,2,3 and 6). All control laboratory animals of both species died after challenge with Salmonella typhimurium virulent strain. In guinea pigs the survival rate was detected higher (70%) in groups (1 and 5) , while it was (60%) in groups (2 and 6) and ( 40%) in groups (3 and 4). On the other side the survival rate in mice groups was recorded higher (80%) in group (5) , (75%) in groups (1 and 2) , (65%) in group (6), while it was (60% and 55%) in groups (3and 4) respectively. The isolation of typhimurium was recorded negative (-ve) in guinea pigs in groups (1,5 and 6) and in mice in groups (1,2,5 and 6). The control group in both species gave high positive result (++++ve).

Publication year 2007
Pages 181-195
Organization Name
City Cairo
serial title the journal of the egyptian veterinary medical association
Author(s) from ARC
Agris Categories Animal diseases
Publication Type Journal
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