Preparation and Characterization of Canine Parvovirus Egg Yolk (Igy) conjugated with FITC and Horse Radish Peroxidase (HRP)
Abstract: The present work could be considered a successful trail to prepare chicken egg yolk IgY against canine parvovirus (CPV) conjugated with horse radish peroxidase (HRP) and fluorescen isothiocyanate (FITC) as local products for diagnostic purpose of CPV infection. Indirect ELISA showed that the induced CPV antibodies in the sera and egg yolk of immunized hens were of high levels with OD of 1.153 and 1.144 respectively. Direct ELISA revealed that the prepared CPV-Igy conjugated with HRP showed positive results up to a dilution of 1:105 and the FITC conjugate showed strong positive apple green reaction up to a dilution of 1:104 and less strong positive results up to 1:106 . So the obtained preparations are suitable of good quality and high specificity to detect CPV antigen.
Publication year 2009
Pages 42-47
Organization Name
City الزقازيق
serial title zagazig veterinary journal
ISSN 1110-1458
Author(s) from ARC
External authors (outside ARC)
    ماجد منير ابراهيم المعمل المركزي للرقاابة علي المستحضرات الطبية
    الهام أحمد الأبياري معهد بحوث الأمصال و اللقاحات
    سيد بيومي الأبياري المعمل المركزي للمستحضرات الحيوانية
Agris Categories Animal diseases
Publication Type Journal
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