Preparation and evaluation of lumpy skin disease hyperimmune serum conjugated with fluorescin
Abstract: Hyperimmune serum against lumpy skin disease virus (LSDV) was prepared by inoculation of the virus in successive doses into susceptible two calves with complete and incomplete Freund’s adjuvant. Identity tets were applied by agar gel precipitation test (AGPT) and indirect fluorescent antibody technique (IFAT). Serum neutralization test (SNT) and ELISA tests were applied for titration and evaluation of the hyper immunesera before conjugation with flurescent. Total protein concentration of the prepared LSDV antisera was 0.89 g/dl. Separation of anti-LSDV immunoglobulins IgG were done using ammonium sulphate followed by conjugation with flurescin isothicyanate at pH 9.6. The ant-LSDV IgG conjugated fluorescin sterile and was used to detect LSDV in the MDBK cells and gave good results to dilution 1/20 while the refrence conjugate to 1/30.
Publication year 2008
Organization Name
City القاهرة
serial title Egyptian journal of virology
ISSN 1685-1687
Author(s) from ARC
External authors (outside ARC)
    ماجدة محمد سيد المعمل المركزي للرقابة علي المستضرات الحيوية البيطرية
Agris Categories Animal diseases
Publication Type Journal
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