Quantification of capripox purified viral proteins
Abstract: Sheep pox “SP” goat “GP” and lumpy skin disease were propagated and titrated individually in Vero or MDBK cell culture and their titres were 5.6, 5.3 and 5.2 log10 TCID50 /ml respectively. The viral proteins of these viruses were estimated by Bradford and Lowery techniques using spectrophotometer. The first technique depends on standard curve while the second based on Lowery equation. The quantity of viral proteins 0.306, 1.440 and 2.074 mg/ml for SPV, GPV and LSDV respectively by using Lowery techniques, while in Bradford technique, the viral proteins were 0.287, 1.297 and 1.986 mg/ml for SPV, GPV and LSDV respectively. The results in Lowery technique were more sensitive and accurate than those obtained by Bradford technique.
Publication year 2009
Organization Name
City كفر الشيخ
serial title the third scientific congress
Author(s) from ARC
External authors (outside ARC)
    محمد سعيد واصل المعمل المركزي للرقابة علي المستحضرات الحيوية البيطرية
    ماجدة محمد سيد المعمل المركزي للرقابة علي المستحضرات الحيوية البيطرية
    محمد أحمد احمد سعد
Agris Categories Animal diseases
Publication Type Conference/Workshop
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