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Immunological study on lumpy skin disease vaccines
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Abstract: Eight cattle were divided into 3 groups, the 1st group of 3 animals was inoculated intradermally (I/D) with 1 ml of alive attenuated lumpy skin disease virus (LSDV) vaccine and the 2 nd group of 3 animals was inocuolated subcutaneously (S/C) with 5 ml of an inactivated LSD vaccine, while the 3rd group of 2 animals was kept as non-vaccinated control. Cell mediated immune response of the vaccinated animals was evaluated by lymphocyte transformation assay. The level of lymphocyte increased from the 1st day post vaccination for both vaccines but the live attenuated vaccine induced a higher level than that induced by the inactivated one. Serum neutralization test and enzyme linked immunosorbent assay were used for evaluation of the humoral immunity. The neutralizing antibodies appeared to be protective on the 14th day post vaccination then increased gradually reaching the maximum level by 28th day post vaccination with the live and inactivated vaccines, respectively, then decreased gradually. The protective level of the antibodies remained till the end of the experiment (180 days) for the attenuated vaccine but it remained only for 60 days in case of the use of inactivated vaccine. The results of the ELISA were parallel and related to those obtained by SNT more sensitive.
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| Publication year |
2007
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| Pages |
14-19
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| Organization Name |
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| City |
cairo
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| serial title |
zagazig veterinary journal
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| ISSN |
1110-1458
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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| Publication Type |
Journal
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