CONJUGATED LINOLEIC ACID: PREPARATION AND BIOLOGICAL EVALUATION

Abstract: Conjugated linoleic acid (CLA) was successfully prepared using three different substrates; IJ dehydration of castor bean oil; 2J isomerization of the separated fatty acids (SFA) after saponification of oil rich in linoleic acid (LA) content and 3J isomerization of commercial linoleic acid. Dehydration process was carried out at 250±5°C for several hours using phosphoric acid (0.1 % w/w) as a catalyst, at with, satisfactory proportions of CLA were obtained. By monitoring the changes in fatty acids composition and other analytical parameters, the highest yield (50.93%) of CLA was attained after only 3 hr of dehydration process. The SF A rich in LA were subjected to isomerization reaction which was achieved at 120±5°C for 48 hr using alkaline catalyst;( sodium hydroxide in n-butanol) under gentle stream of nitrogen. Commercial linoleic acid was also subjected to alkaline isomerization following the same conditions. F As obtained after alkaline isomerization from both process were methylated to fatty acid methyl esters (FAME) and analyzed by gas chromatography (GC). Data showed that 24 and 48 hr of isomerization were sufficiently enough to produce a satisfactory amount of CLA from LA and SFA (75.74, 77.67%), respectively. CLA produced after 24 hours of continuous isomerization of linoleic acid (75.74%) was examined for potential cytotoxicity by SRB assay. Results revealed that the prepared CLA exhibited an efficient cytotoxicity against breast carcinoma cell line (MCF7) with ICso value of 2.42J.lg.
Publication year 2009
Pages 23 - 38
Organization Name
serial title Minia J. ofAgric. Res and Develop
Author(s) from ARC
External authors (outside ARC)
    سماح سعيد علام Oils & Fats Res. Dept., Food Techno!. Res. Inst., Agric. Res. Center, Giza, Egypt
Publication Type Journal
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