PHYTOCHEMICALS CHARACTERIZATION AND ANTICANCER ACTIVITY OF EGYPTIAN PURSLANE
Abstract: Oxidized unsaturated fatty acids may contribute to the pathogenesis of atherosclerosis. The aim of the present study is to investigate the effect of cardamom, cinnamon and ginger powder at (7%) on oxidative stress in rats fed on oxidized oil. Sunflower oil was submitted to fryer treatments at temperature between (180-190 °C) on intermittent periods for 24 hrs. The changes in chemical properties of frying oil compared to fresh sunflower oil were investigated. Data illustrate the presence of an increase in acid value, peroxide value, polymers and a decrease in iodine number. Fatty acids constituents of this tested oil were also investigated. Forty-two rats weighting approximately 200 grams were divided into seven groups, each group containing six rats. Group S1 was fed basal diet and kept as negative control. Groups S2 and S3 fed on diets containing oxidized oil (15 %) and the other groups S4, S5, S6 and S7 fed on the same diet used in group S2 and supplemented with different antioxidants cardamom ,cinnamon ,ginger and mixture powder (7 gm/100 gm diet ), respectively. Rats fed oxidized oil (S2 and S3) had higher concentrations of cholesterol, LDL-cholesterol and triglycerides. The damage caused by the oxidized oil was revealed by a significant decrease in enzymatic antioxidants, (SOD), catalase and (GSH-Px) in blood and significant rise of malonaldehyde (MDA) in serum of rats in groups S2 and S3 compared to other groups (S4, S5, S6 and S7). Since the study of induction of the redox enzymes is considered to be a reliable marker for evaluating the antiperoxidative efficacy of the spices, these findings suggest a possible antiperoxidative role. Histopathological studies showed reduction of the damaged tissue in the heart caused by feeding on the oxidized oils. Hence, these spices may be used for preventive and therapeutic purposes.
Egyptian purslane (Portulaca oleracea L.) is a green plant which has been known since times of the ancient Egyptians rarely found now in Egypt. The objective of this work was to identify the phytochemical components of purslane leaves and seeds. Samples were analyzed for their chemical composition, mineral nutrient composition, fatty acids (FA), total phenols content (TPC), total flavonoids contents (TFC). The scavenging effect of DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals was evaluated, Also, the cytotoxic activity of purslane leaves and seeds against human liver cell line (HEPG2 0.571) was studied. The study showed that purslane is rich in natural minerals, antioxidants, and omega-3 fatty acids. Significant differences were observed in a few of the recipes in regard with the sensory attributes (Taste, odor, texture color, Appearance and overall acceptability) of purslane recipes with that of control (Spanish plant). It can conclude that purslane is an excellent source of bio-active components which has biological impacts on human health and could be used .This work can helps to create an interest Egyptian dish form purslane as a functional food used for nutraceutical applications.
Key words: Antioxidant - Chemical evaluation - Cytotoxic activity- Egyptian Purslane plant - Phenolic profile and Phytochemical components.
Publication year 2016
Pages 75-103
Organization Name
serial title J. Biol. Chem. Environ. Sci.
Author(s) from ARC
Publication Type Journal
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