Effect of Low and High Esterified Pectin on Arsenic Toxification in Rats

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Abstract: The main purpose of the present study is to evaluate the efficiency of each low and high esterified pectin on arsenic
absorption, retention and removal in male albino rats (Sprague Dawley strain). Fifty male albino rats were divided into ten groups (5rats for each), the first group was fed on based diet (b d) as a (-)control group (NC), groups (2-4) received b d with 4.0, 8.0 and 16 mg/Kg sodium arsenite (SA) /Kg diet daily as which represents 1/100,1/50 and 1/25 from sodium arsenite, LD50 in the diet as control (+) 1,2 and 3 respectively, groups (5-7) , received bd with 8% high esterified pectin (HEP, DE 73.5) with the previously SA doses and groups (7-10), received bd with 8% low esterified pectin (LEP, DE 31%), and the same SA doses. Rats were fed with LEP with 4.0 mg SA/Kg diet resulted in the best improvement of the nutritional valus in addition to the histopathological changes. At the end of the experiment (30 day) the mean value of serum alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), uric acid, urea, creatinine and lipid peroxide (TBARS) decreased in the groups were fed HEP or LEP in the presence of the previously SA doses when compared with the same positive control. The best results of the previously parameters obtained in group was fed with 8% LEP with 4.0 mg SA/Kg diet except ALT which obtained with 8.0 mg SA/Kg diet when compared with negative control group. These results were (23.92 VS. 24.08 U/L), (102.3 vs. 82.44 U/L), (120.9 VS. 110.5 IU/L), (4.19 VS. 3.79mg/dL), (13.46 vs. 14.05 mg/dL), (0.56 vs. 0.43 mg/dL) and (6.17 vs. 6.28 n mol/ml) respectively. On the other side, total protein and albumin were increased in all groups fed with HEP or LEP in the
presence of all previously concentration of SA. Maximum improvement of total protein and albumin were obtained in the group fed with LEP with 4.0 mg SA/Kg diet when compared with negative control which amounted (7.19 vs. 7.21 g/dL) and (4.1vs. 4.6 g/dL),respectively. On the other hand, arsenic retention in kidney, brain and liver reduced in all groups were fed with HEP or LEP as compared with positive control. The highest removal of arsenic from the previouslyorgans specially in liver were obtained in the group was fed with LEP with 4.0 mg SA/Kg diet when compared with negative control which amounted (0.532 vs. 0.000155 ppm), (0.317 vs. 0.000075 ppm)and (0.074 vs. 0.000009 ppm) respectively. On contrast, theconcentration of arsenic exerted in feces increased gradually by increasing the doses presented in the diet. Maximum arsenic exerted in feces was in the group was fed 8% LEP with 16 mg SA/Kg diet which amounted 8.549 ppm. The histopathological examination is in good agreement with biochemical measurements. It could be recommended from these results that LEP as a powerful bindmaterial of arsenic should be incorporated into human foodconsumption daily to reduce the hazard toxicity of arsenic pollutionof food and water.

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Publication year

2008

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معهد بحوث تكنولوجيا الاغذية - ش جامعة القاهرة - الجيزة

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Organization Name

Food Technology Research Institute (FTRI)

serial title

J. Biol. Chem. Environ. Sci.