Incorporation of Moringa leaves extract in Pectin-based edible coating as antimicrobial agent
Abstract: It is become very necessary to look for new antimicrobial substances from other sources such
as plants, which the leading important things about utilizing plant-derived antioxidant are largely less
dangerous than artificial ones and provide deep restoration benefits. The present study was carried out
to evaluate the potential of edible pectin film that contains Moringa oleifera leaf extract as a new
biodegradable film maintaining the shelf life of food and improve its microbiological safety. In this
study, bioactive compounds of moringa were extracted by using varied solvents; ethanol 70%, acetone
95% and water. The three extracts were analyzed to determine polyphenols, flavonoids content and its
antioxidant activity. Also the extracts were screened for in vitro antimicrobial potential against some
probiotic bacteria strains (E-coli 0157: H7, B. Cereus, S. aureus, P. aeruginosa and L. plantarum) at
different concentration (50, 75, 100, 150 and 200 mg/mL-1) of moringa extract in dimethyl sulfoxide
solution. Four concentration of glycerol (0.5, 1.0, 1.5 and 2.0%) were investigated as plasticizer agent
in moringa extracts-containing pectin films to optimize the permeability to gases and mechanical
properties of them. Moringa extracts-containing pectin films were evaluated by measuring optical &
mechanical properties and its microbiological activities against E. coli 0157: H7 and S. aureus at
varied concentration of solvents extract (0.3, 0.5 and 1.0%). The results demonstrated that moringa
leaf ethanol extract, between the other solvents, had a higher amount of total phenolics (158.79 mg
GAE/g), flavonoids (66.37 mg QE/g) and strong antioxidant activity (82.47%). Also results revealed
that 200 mg mL-1 moringa leaf ethanol extract had maximum zone of inhibition against the pathogenic
strains used in this study which ranged between 15.34 – 18.27 mm. The film formula of 2.0% glycerol
was the best mechanical properties of it. The date confirmed that with the increase of moringa extract
concentration, there are similar increase in TS, E% and decrease in O2P and WVP of the pectinmoringa
film and OP improved at the same time in addition, the films exhibited a clear antimicrobial
activity against E.coli 0157: H7 and S. aureus with the increasing of moringa extract concentration.
Thus, moringa is extremely valued plant, with impressive content of bioactive compounds, therefore,
ME-containing pectin film can be used as antimicrobial and antioxidative packaging materials to
improve the quality of food products and to prolong its shelf life.
Publication year 2018
Pages 602-615
Availability location 2077-4435
Availability number
Organization Name
Author(s) from ARC
Publication Type Journal
↑