Preparation Two Specific Stained Antigens For The Detection Of Escherichia Coli O157:H7 Infection And Bru Cellosis
Abstract: Escherichia coli 015 7 antigen share elements with Brucella smooth strains which cause cross reactivity in serological standard tests for Brucellosis. In infected animals with Escherichia coli O 157:H7 , antibodies against E. coli cross react with the Brucella antigens (Strain 99 Rose Bengal Test). Consequently, Brucellosis is difficult to diagnosis because shared with E. coli 0157:H7. The aim of this study was to elaborate on a method of antigen preparation that is useful in detecting specific antibodies against each bacterial strain without cross reactivity. In this study, sera were examined from animals that are: vaccinated with Brucella vaccines (Rev.1 and S19); natura1ly infected with Escherichia coli 0151:H7 and Brucellosis; and experimentally infected with Escherichia coli 0151:H7, wild field Brucella melitensis, and other E. coli serotypes. All those sera were ex-amined for antibodies against the developed two antigens. Cross reaction disappeared.. E. coli 0157:H7 antibodies failed to react with the modified Brucella antigen and Brucella antibodies failed to react with the colored antigen of E. coli 0151:H7 .In effect, we were able to demon-strate mixed infection and prove the effectively of the developed antigens. This implicitly proves that the developed antigens have a high degree of specificity and sensitivity. They could be a useful tool for clinical diagnosis and epidemiological studies of E. coli 0151:H7 in-fection and Brucellosis in animals with potential use in humans.

Keywords: Bacterial antigens, bacterial cross reaction. Brucellosis, Enterohaemorrhagic Escherichia coli, TEM (Transmission Electron Microscopy)
Publication year 2008
Availability location مركز القاهرة الدولى للمؤتمرات
Availability number
Organization Name
City القاهرة
serial title مؤتمر الشرق الأوسط وشمال أفريقيا لبحوث الثروة الحيوانية
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Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Brucella.
Publication Type Conference/Workshop
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