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PURIFICATION AND CHARACTERIZATION OF PEROXIDASE FROM DATE PALM (PHOENIX DACTYLIFERA L.) CALLUS CV. BARHEE
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Abstract: Peroxidase (POD, E.C 1.11.1.7) is one of an enzymatic defense system against active oxygen species (AOS) in date palm (Phoenix dactylifera L.), especially during callus stage. This study aimed to extract peroxidase (POD) from date palm (cv. Barhee) callus tissue for biochemical characterization. In the crude extract of callus peroxidase activity reached (161.14 U). Partially purified POD with 30% ethanol reached 7.14 purification fold, whereas it increases with Sephadex G-100 to reach 61.77. The molecular weight of purified POD was 43 kDa while the optimum pH and temperature were 6.5 and 40°C, respectively. Kinetic constants Km and Vmax for guaiacol was 0.4 mg/reaction and 0.87U.min-1, respectively while for H2O2 was 0.24 mM/reaction and 0.44 U.min-1. Browning phenomenon in Barhee callus could be referred to the increase of POD activity always accompanied with phenolic compounds oxidation. Determination of optimum conditions for POD from Barhee date palm callus could control the browning phenomenon in additions could be a source as a natural product from Barhee date palm callus for another application.
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| Publication year |
2019
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| Publication Type |
Journal
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