Efficiency of Rhizobum japonicum strains as affecting nitrate reductase and dehydrogenase activities of the soybean nodules

Abstract: Seeds of the soybean, variety Clark, when planted in Nile silt soil and inoculated with different strains of R. japonicum. After 59 and 87 days of growth, plants were uprooted, analised for dry weight, total nitrogen, fresh weight of nodules, nitrate reductase activity in the leaves, and nitrate reductase and dehydrogenase activities in the nodule homogenates in the presence or absence of succinate, citrate, ethyl alcohol and total phenols. The data were analyzed to produce the correlation coefficients between total nitrogen and other characteristics.
A significant positive correlation was evident between nitrogen fixation and weight of pods. The phenol content of the nodule homogenate and the nitrate reductase activity in the leaves were negatively correlated with the nitrogen fixation. The nitrate reductase activity in the leaves decreased with age of the plants.
The dehydrogenase and the nitrate reductase activities in nodule homogenate gave no significant correlation with nitrogen fixation. However, the leaves of their activities differed considerably with strains. An increase in the general activity of the dehydrogenase was accompanied by a decrease in the general nitrate reductase activities. The succinate dehydrogenase was the most active in the nodule homogenate, formed by strains 514, 138, and E45. The citrate dehydrogenase was more active with strains 513 and E41. Ethanol dehydrogenase or the endogenous dehydrogenases were the least in all nodules, caused by all strains. The nitrate reductase activity was not affected by different hydrogen donors. The hishest nitrate reductase activity was observed in the nodule homogenate of strain 138. In general, no particular correlation between nitrogen fixation and dehydrogenase and nitrate reductase activities in nodule homogenate was found .
Publication year 1978
Pages pp. 414-420
Organization Name
serial title Zbl, Bakt. ΙΙ. Abt., Bd..
Author(s) from ARC
Agris Categories Weeds and weed control
AGROVOC
TERMS		 Nitrate reductase inhibitors. Soybeans.
Proposed Agrovoc Glycin max;Rhizobum japonicum;soybean nodules;dehydrogenase a;
Publication Type Journal
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