The metabolism of 2,4 – dinitro – 0 – sec . butylphenol (dinoseb) by a soil micro-organism

Abstract: A micro – organism was isolated after a lage of 4 weeks from a soil sample
Perfused with 2, 4 – dinitro – o – sec . butylphenol ( 0 . 01 % ) mineral salts midia
And found capable of utilizing this compound in pure liquid cultures . Only a slow
Degradation of dinoseb was observed when the organism was inoculated into liquid
Media containing this herbicide as a sole source of carbon . Faster degradation ,
However , was obtained in the presence of hydrogen – donors ( 0.5 % w / v
Glutamate , or lactate , succinate , yeast extract or nutrient broth ) . During the
Growth of such cultures the yellow colour of dinoseb changed gradually to a deep
Red colour . At this point 100 % of the compound had disappeared and its peak at
370 nm was completely replaced by another at 460 nm . After reaching a maximum
value , the deep – red colour disappeared gradually , leaving a yellow – tinged culture
Chromotographic analysis of the acid – ether extracts of cultures showed the
Presence of a major intermediate with X max . at 465nm . This compound was
Isolated as the O : N dibenzyol derivative and identified as 2 – amino –4 – nitro
O – sec . butylphenol .
Publication year 1974
Pages 18
Availability location (المعمل المركزى لبحوث الحشائش) 9ش الجامعة جيزة.
Availability number
Organization Name
serial title Society of Applied Microbiology Arab Republic of Egypt March 30-31 st
Author(s) from ARC
Agris Categories Miscellaneous plant disorders
AGROVOC
TERMS
Degradation. Metabolism. Soil microorganisms.
Publication Type Conference/Workshop