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Sensitivity of Different Methods Used In Determination
of Formalin Residues in Inactivated Veterinary Vaccines
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Abstract: Formaldehyde concentration is very crucial in the potency of inactivated vaccines and its adverse
effects. High level of formaldehyde will affect on potency of the vaccine by masking the B and T cell epitopes.
Determination of formaldehyde concentration can be measured by visual method (phenyl hydrazine) and by
spectrophotometric methods (phloroglucinol and ferric chloride methods). In the current study, the sensitivity
and specificity of these methods were investigated. The visual method is not accurate where it is depended on
naked eye in matching of a coloured complex product while the two spectrophotometric methods gave nearly
the same values and required inexpensive instrumentation(spectrophotometer) with simple operation. The ferric
chloride quantitative colorimetric method depends on complicated principle, easily used in routine analysis and
the used chromogenic agent is fairly expensive. In addition, this method is highly sensitive and accurate
compared to phloroglucinol method where it could determine the formaldehyde concentration of all inactivated
veterinary vaccines either bacterial or viral. Also, it could differ between the oil adjuvanted and the gel
adjuvantedinactivated vaccines during the operating process.
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| Publication year |
2015
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| Pages |
546-552
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| Organization Name |
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| Author(s) from ARC |
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| Publication Type |
Journal
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