heterosis, combining ability and biochemical genetic markers for salt tolerant genotypes in grain sorghum
Abstract: Three cytoplasmic male sterile (A-lines) i.e. ICSA37 (f1), ICSA88015 (f2) and ATX631 (f3) and four restorer lines (R- lines) i.e. ICSR89016 (m1), ICSR89025 (m2), ICSR89038 (m3) and ICSR 90015 (m4) and their twelve hybrids of grain sorghum [Sorghum bicolor(L.)Moench]were evaluated under normal and saline soil conditions for days to 50% heading, plant height, leaf area, 1000 kernel weight and grain yield per plant at Nubaria Agric. Res. Stat., in 2004 growing season. The genetic analysis was conducted using line x tester analysis. The obtained data revealed highly significant differences between environments for all studied traits. Mean squares due to genotypes, crosses and parents were significant for all studied traits under normal and saline conditions except 1000 kernel weight for crosses and parents under normal conditions. Mean squares due to interaction of genotypes with environments were highly significant for all studied traits except for 1000 kernel weight, indicating inconsistent response of these genotypes from normal to saline conditions. Variances due to the interaction among each of male general combining ability (GCA), female GCA and specific combining ability (SCA) with environments (E) were significant for all studied traits except male GCA x E for leaf area, female GCA x E for leaf area and 1000 kernel weight and SCA x E for 1000 kernel weight and grain yield per plant, which were insignificant. This indicates that, both additive and non-additive gene effects were influenced by environments.
Salinity susceptibility index (SI) indicated that, the best salt tolerant hybrids were f2 x m2, f2 x m3, f2 x m4, f1 x m2, f1 x m3 and f3 x m3 and the best salt tolerant parental lines were f3, f1 and m2. Heterosis over better parents showed that, the best hybrids under normal and saline conditions were f2 x m2, f2 x m3 and f1 x m3 for grain yield per plant and some of the other studied traits. The best general combiner for grain yield per plant and some of the other studied traits was the female line f2 under saline conditions. The best SCA hybrid was f2 x m2 under both normal and saline conditions for grain yield per plant and some of the other studied traits. SDS protein banding pattern under saline conditions showed that, the tolerant grain sorghum genotypes are distinguished with nine common bands which were not found at the sensitive genotypes at MW of 245.31, 219.35, 140.66, 100.57, 67.22, 62.47, 58.13, 36.19 and 33.57 KDa. In general, the electrophoretic patterns of leaf total proteins could be a useful tool for the identification and characterization of the tolerant grain sorghum genotypes.
Publication year 2006
Pages 117-130 (
Availability location معهد بحوث المحاصيل الحقلية 9- شارع الجامعة- الجيزة
Availability number
Organization Name
serial title المجلة المصرية لتربية النبات:
Author(s) from ARC
Agris Categories Agriculture - General aspects
AGROVOC
TERMS		 Heterosis. Sorghum.
Publication Type Journal
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