Tissue Culture Organogenesis in Soybean
Abstract: ABSTRACT

The aim of this study was to initiate and maintain callus organogenesis cultures of soybean and evaluate the response of seven soybean genotypes in callus formation. The genotypes were selected on the basis of their reaction to cotton leaf worm infection. This investigation was conducted in the field at Giza research station in 2003 and 2004 season. Immature embryos (with 0.5-10 mm long) were excised from immature seeds and used as ex-plant. The basal murashing and skoog (MS) medium with addition of 4 times of micronutrients, proline (1381 mg/l), NAA (0.0372mg/l) and BAP (2.996 mg/l) gave complete satisfaction for callus production. Formation of shoots and roots were succeeded and rooted plantlets were transplanted pots in green house. The results indicated that the genotype L 86 K – 7 3 was the best in response to tissue culture technique in soybean. Callus growth rate was positively and significantly correlated with each of number of shoots/callus and number of roots. Therefore. Both characters could be used to predict succeeding of callus growth in soybean.

Publication year 2005
Pages 5201-5208
Availability location معهد بحوث المحاصيل الحقلية - مركز البحوث الزراعية - ش الجامعه - الجيزة
Availability number
Organization Name
serial title J. Agric. Sci. Mansoura Univ., 30 (9): 5201-5208,
Author(s) from ARC
Agris Categories Agricultural researchPlant genetics and breeding
AGROVOC
TERMS		 Organogenesis. Soybeans. Tissue culture.
Publication Type Journal
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