Metabolism of p-Hydroxybenzoic Acid by Certain Strains of Rhizobia
Abstract: R . phaseoli strain 405 and R . japonicum strain E 41 ( kindly supplied by Dr. O. N. Allen, Wiscons., and Mr. E . Sheil Argentina) were grown in the following medium ( g . / I . ) : KH2PO4, 1.0; NaH2PO4 . 2H2O, 2.36; MgSO4 . 7H2O , 0.1; NaCI , 1.0; CaCI2 . 2H2O, 0.13; FeSO4 . 7H2O, 0.005; Na – glutamate, 0.5. p- hydroxybenzoic acid was added to give a final concentration of 10- 3M. The culture of R . phaseoli 405 was grown for 5 days at 25 C, that of R . japonicum E 41 was incubated for 15 days at the same temperature At the end of the incubation period the cells were harvested by centrifugation at 3500 r.p.m . for 20 minutes, washed, and suspended in a phosphate buffer 0.1 M , pH 7. The cells were kept at 4 C up to their use .
The normal Warburg technique (using Warburg appartus V 166, Messrs. Braun Melsungen) was used for measuring the O2- consumption of the cells of the two rhizobia strains in the presence of 10-3 M of catechol . p-hydroxybenzoic acid, and protocatechuic acid .
These results confirm our earlier suggestion that the metabolic pathway of P –
Hydroxybenzoic acid in R. phaseoli 405 proceeds through protocatechuic acid, and
in this report also in the R. japonicum E 41. It is further indicated that the proto catechuic acid ring is cleaved between the two hydroxylated carbon atoms, giving rise to B- Ketoadipic acid.
Publication year 1975
Pages 34-36
Availability location المعمل المركزي لبحوث الحشائش - مركز البحوث الزراعية - ش الجامعة - الجيزة
Availability number
Organization Name
Author(s) from ARC
External authors (outside ARC)
    Y . A . H ussein
    Y . A . Hamdi
Agris Categories Pests of plantsWeeds and weed control
AGROVOC
TERMS		 Metabolism. Pests of plants. Rhizobiaceae.
Publication Type Journal
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