Genetic Diversity of Egyptian Barley Using Agro–Physiological Traits, Grain Quality and Molecular Markers
Abstract: Assessment of phenotypic and genotypic diversity is one of the principal and important steps in plant breeding programs. In this study, field screening analysis was carried out in Sakha Agricultural research station during two growing seasons 2018/ 2019 and 2019/ 2020 to investigate the phenotypic diversity among 15 Egyptian barley cultivars using physiological, grain quality, grain yield and yield-associated traits and to assess the genetic diversity by using Sequence-Related Amplified Polymorphism (SRAP) marker analysis. Analysis of variance of the traits showed high variability among all cultivars under study with respect to all agronomic and grain quality characteristic studied traits. Cluster analysis classified the cultivars in four groups and showed that genetic variation based on the all studied traits among the barley cultivars. Ten SRAP combination primers was used, the average percentage of polymorphic loci of the 67.9 % and the average band number amplified from each pair of primers was 6.5% bands, of which included 9.0 % polymorphic bands. Highest (PIC), was related to primer me6+em5 was (0.94) indicating that this primer is highly informative. The dendrogram of SRAP markers had clustered all the Egyptian cultivars into four groups each group include the most closed cultivars. The results of the present study showed that there were high genetic information differences among Egyptian barley cultivars which offered new information about the genetics relationships between Egyptian barley cultivars which they are useful for cultivar identification and for their utilization in further barley programs for environments stress.
URL
Publication year 2021
Pages 58-71
Organization Name
Web Page
Author(s) from ARC
Publication Type Journal
↑