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Detection and Molecular Characterization of Ralstonia solanacearum Race 3 biovar2 isolates, Using Insertion Sequence (IS) Analysis in Egypt
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Abstract: The pathogenic and genetic variations of Ralstonia solanacearum, the causal agent of potato wilt, make the development of diagnostic, detection, and control measures of R. solanacearum more difficult and complex. The variation among 48 Egyptian isolates of Ralstonia solanacearum recovered from different habitats was assessed on the basis of pathogenicity and the existence of genomic insertion sequence (IS) using polymerase chain reaction (PCR) fingerprint method. IS was determined in all tested isolates by using PCR technique that revealed a distinguished pattern which could be used as a genetic marker for rapid identification of R. solanacearum R3B2 isolates. Moreover, the genetic variation among isolates of R3 B2 was studied by detecting the presence/absence of miniature inverted repeat transposable elements (MITEs) in their genomic structure. The use of IS was necessary for detection/identification of isolates, while the possible relations between pathogenic potential and genetic diversity of R. solanacearum (R3 B2) were correlated using MITEs detection.
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| Publication year |
2011
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| Pages |
154-168
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| Organization Name |
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| serial title |
Egypt. J. Phytopathol
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| Author(s) from ARC |
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| Publication Type |
Journal
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