Induction of disease defensive enzymes in response to treatment with acibenzolar-S-methyl (ASM) and Pseudomonas fluorescens Pf2 and inoculated with Ralstonia solanacearum race 3, biovar2 (phylotype II)

Abstract: Ralstonia solanacearum (Rs) race 3 biovar 2, the causative agent of bacterial wilt is an economically important pathogen in tropical, subtropical and temperate regions of the world. This study investigated the induced defense responses against tomato bacterial wilt by the application of acibenzolar-S-methyl (ASM) and Pseudomonas fluorescens (Pf2) alone or in combination. Seedling treatments of tomato plants with either Pf2 or ASM significantly reduced the disease severity of bacterial wilt (58 and 56% disease reduction, respectively) on tomato plants. The highest disease reduction (72%) resulted from a combined application of both Pf2 and ASM. The application of ASM alone increased seedlings biomass relative to infected control with 64.3%. Changes in the enzymes activities of Polyphenoloxidase (PPO), ?-Glucosidase (?-
GL) and Perioxidase (PO) on tomato after the application of ASM and Pf2 and inoculation with Rs were studied. Significant changes (p ? 0.05) in the activities of PPO, ?-GL and PO were found. Our results indicate that the future integrated disease management programs against tomato bacterial wilt may be enhanced by including foliar sprays and soil drench of ASM and P. fluorescens. This is the first report of the use of both ASM and Pf2 to control the tomato bacterial wilt disease under field conditions.
Publication year 2012
Pages 382–389
Organization Name
serial title Journal of Plant Pathology
Author(s) from ARC
Publication Type Journal
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