Observation on anatomical changes in guava leaf (Psidium guajava L.) infected with Colletotrichum gloeosporioides Penz and Sacc. , the causal agent of anthracnose during host pathogen interaction
Abstract: Abstract:
Anthracnose, caused by Colletotrichum gloeosporioides, is a foliage, pre and post harvest disease of guava. Artificially inoculated leaves by C. gloeosporioides were performed to understand the infection processes of anthracnose at various intervals after inoculation compared with naturally infected were performed. The first evidence of penetration into the leaf was observed 48 h after inoculation, fungal hyphae colonized epiderms tissues through cuticle layer and spread inter and intracellular with evidence of cell cell plasmolysis, disorganization and dark brown discoloration of some parenchymatous cells (P). In advanced stages, seventy two hours after artificial inoculation (hai) the pathogen spread to adjoining invaded tissues causing complete breakdown of the inoculated tissues and hyphae (h) extensively ramified in the invaded intercellular spaces, endodermal cells and xylem units also, mycelia were also present in and within the xylem (Xy) elements. Transfer sections of After 96 h, all the invaded cells became necrotized and vascular bundle was completely plugged with gum like substances. No differences were found between naturally infected and artificially inoculated (in vitro) leaves , the pathogen invasion and symptom development process is similar for both conditions. C. gloeosporioides is able to invade different tissues causing changes in cells structure and caused plasmolysis and discoloration of parenchymatous cells as well as necrosis in different leaf tissue. These necrotized tissues increase with the time of inocubation.

Key wards: Anatomical changes, guava anthracnose disease, Colletotrichum gloeosporioides
Publication year 2014
Pages 22-33
Organization Name
Author(s) from ARC
Publication Type Researsh & Applied Activities
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