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Role of guava phyllospheric bacterial isolates in controlling leaf and fruit anthracnose caused by Colletotrichum gloeosporioides
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Abstract: Abstract:
Biocontrol activity of 277 bacterial isolates obtained from guava leaf phyllospher were screened for an antagonistic effect against Colletotrichum gloeosporioides Penz. in dual culture. About 21.6% of all isolates tested exhibited 50% less inhibition growth. Only 8.3% of the isolates providing inhibition greater than 50%. While, five isolates reduced the growth diameter of the fungus by more than 70%. The highest antagonistic bacterial isolates were identified as Bacillus subtilis (isolates, WBs1& WBs7) and Bacillus pumalis ( isolates, WBp15, WBp17and WBp25) based on morphological, biochemical and physiological properties . Isolates, WBs1& WBs7 provided the highest efficacy for reducing percentage of spore germination and length of germ-tube over control. Both B. subtilis isolate (WBs1& WBs7 ) and isolate, WBp15 of B. pumalis released chitinase when grown in mixture culture with C. gloeosporioides. Isolate, WBp15 of B. pumalis showed the highest chitinase activity. No chitinase activity were detected in the rest isolates. On the other hand, all the isolates tested produce β-1,3 glucanase. B. subtilis (isolate, WBs1) recorded the highest amount of β-1,3 glucanase while the lowest one was WBp17of B. pumalis. All candidate antagonistic of bacteria isolates could reduce disease severity on guava leaves and fruits, when applied at 24 h before inoculation of C. gloeosporioides as spray application than after inoculation.
Key words: Guava - anthracnose – Colletotrichum gloeosporioides- Bacillus pumalis - Bacillus subtilis – spore germination.
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| Publication year |
2012
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| Pages |
199 – 211
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| Organization Name |
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| serial title |
J. Biol. Chem. Environ. Sci
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| Author(s) from ARC |
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| Publication Type |
Journal
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