Low-cost fermentative medium for microbial production of exopolysaccharides from Lactbaciluskefiranofaciens and yeast strain
Abstract: Deproteinised whey was fermented with a coculture Lactobacillus kefiranofaciens NRRL Y - 1838 and Saccharomyces cerevisiae EMCC 69 for exopolysaccharides production. The yeast utilizes lactic acid produced by the lactobacilli, thus adjusting the pH for exopolysaccbarides production, which called kefiran, Exopolysaccbaride concentration increased with fermentation.time reachittg values of 220 mg/l after 120 h of fermentationin with 15 % of inoculum (vlv) and 40 °C.
Polysaccharide production was affected by temperature. And maximum production was observed at 40°C at different ratio of L. kefironofaciens and S. cerevisiae. The resultant polysaccharide by L. kefiranofaciens NRRL Y-1838 and S. cerevisiae EMCC 69 consisted of 45% total carbohydrate, 27% ash and content 17.481 % protein.
Increasing shear rate from 55 to 475 s-1 at 25 °C due to decreasing the apparent viscosity of polysaccharide solution at different concentration. A concentration of 1% of polysaccharide increase the apparent viscosity of over a broad range of conditions; a pH 2 to 8, 0 to 3% sodium chloride and 0 to 15% sucrose.
In conclusion L. kefiranofaciens was able to acidify deproteinised whey, reducing lactose concentration increase the producing a soluble polysaccharide.
Publication year 2010
Pages 597-609
Availability location معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى- الجيزة
Availability number
Organization Name
serial title Proceeding of the 11th Egyptian Conference Dairy Science & Technology
Author(s) from ARC
External authors (outside ARC)
    سماح احمد شلبى قسم علوم الاغذية - جامعة عين شمس
    محمد نبيل ابراهيم المجدوب قسم علوم الاغذية - جامعة عين شمس
AGROVOC
TERMS		 Lactobacillus. Lactose. Polysaccharides. Saccharomyces cerevisiae. Whey. Yeasts.
Proposed Agrovoc Lactobacillus kefiranofacie;exopolysaccharides;kefiran;
Publication Type Conference/Workshop
↑