Effects of ascorbic acid and glutathione on in vitro maturation of new Zealand white and Baladi black rabbit oocytes
Abstract: The main objective of this study was to evaluate the effects of level of some antioxidants; such as ascorbic acid (AA) and reduced glutathione (GSH), on in vitro maturation rate of New Zealand white (NZW) and Baladi black (BB) rabbit oocytes. Oocytes were collected from 20 NZW and 20 BB rabbit. Oocytes were immediately subjected after collection to slicing technique. Only compact cumulus oocytes (COCs) were used in this study. Five types of maturation medium were used in this study included TCM-199 supplemented with 2 or 3 mM of AA and 0.5 or 1 mM of GSH as well as control medium (without supplementation). The medium was initially equilibrated in CO2 incubator (5% CO2, at 38°C with saturated humidity) for 60 minutes, then the oocytes were cultured in the medium under the same conditions for 20 h as a maturation period. Thereafter, oocytes were fixed (with 3 ethanol: 1 glacial acid), stained (with 1 % orcein in 45% acetic acid), and microscopically examined. The present results revealed that supplementation with 2 mM AA yielded the highest (P<0.05) percentage of NZW oocytes anested at M-II stage (70.59%), but did not differ significantly from that achieved with the supplemental level of 0.5 mM GSH (67.27%). Supplementation with 3 mM AA gave significantly higher (P<0.05) percentage (62.50%), and supplementation with 1 mM GSH exhibited significantly similar percentage (57.5%) 0'1' NZW oocytes arrested at M-Il as compared to that of their respective control medium (58.73 %). As for BB oocytes, supplementation with 3 mM AA yielded the highest (P<0.05) percentage of oocytes arrested at M-Il stage (79.39%), but did not differ significantly from that (77.5%) obtained with the low AA supplemental level (2 mM).On the other hand, supplementation with either level of GSH (0.5 and 1 mM) failed to improve percentage of BB oocytes anested at M-ll as compared to that achieved with their respective control medium (58.33 and 64.12 vs. 71.76%, respectively). Overall percentage of oocytes arrested at M-II was higher in BB than that in NZW does (70.10 VS.
62.97%). Conclusively, the present results may suggest that the supplementation of TCM-199 with AA had a beneficial effect on the in vitro maturation of rabbit oocytes; and that can be achieved at level of 2 mM for NZW and 3 mM for BB oocytes.
Keywords: Rabbit, NZW, Baladi, ascorbic acid, glutathione, in vitro maturation.
Publication year 2012
Pages 1-15
Availability location معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى- الجيزة
Availability number
Organization Name
serial title Egyptian Journal Basic Applied and Physiology
ISSN 1687-1146
Author(s) from ARC
External authors (outside ARC)
    ترك محمد درة جامعة المنصورة
    عبد الخالق السيد عبد الخالق جامعة المنصورة
    عبد البصير حمزة ريا جامعة المنصورة
Agris Categories Animal physiology - Reproduction
AGROVOC
TERMS		 Antioxidants. Ascorbic acid. Glutathione. Rabbits.
Proposed Agrovoc in vitro maturation;Baladi;NZW;
Publication Type Journal
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