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Studies on the effect of addition of Korean red Ginseng@ aqueous extracts on the fertilizing capacity of rabbit semen
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Abstract: One hundred sixty eight sexually mature Baladi Black (BB) rabbits (26 bucks aged 9 months and 142 monoparous does in two sequence parities) were used in the present study. Semen was collected artificially, pooled and diluted with sodium citrate diluent. Aqueous extracts of Korean red genseng@ were added to the diluted Semen at different concentrations of 0 (control), 25, 50, and 75 μl/ ml, then the diluted semen was divided into two parts, the first part was stored at refrigeration temperature(4 - 6 ºC) for up to 3 days while the second part was incubated at 37 °C for up to 4 hrs. Percentages of sperm motility; alive and normal spermatozoa and acrosomal defects were recorded at the different stages of the preservation. Sperm penetration into estrous cow cervical mucus was estimated during diluted semen incubation. Evaluation of the activities of glutamic-oxaloacetic transaminase (GOT); glutamic _pyruvic transaminase (GPT); acid phoshatase (ACP) and alkaline phosphatase (ALP) enzymes in the BB rabbit semen were measured during preservation. One hundred and forty two rabbit does in two consecutive parities were artificially inseminated using either the diluted semen free or supplemented with 50 μl Korean red ginseng extract/l ml diluted semen (the level which showed the ideal effects on semen quality) to estimate the fertility traits. In both parts of the supplemented diluted rabbit Semen with Korean red ginseng@ extract showed that there was significant improvement in:(P< 0.05) sperm motility, the percentages of alive spermatozoa, normal spermatozoa and storage ability , while the acrosomal defects percentages were significantly (P<0.05) decreased. Sperm penetration into estrous cow cervical mucus were arranged significantly (p < 0.05) in descending order as obtained by supplemented diluted semen with Korean red ginseng@ extract (50, 75, 25 μl/ml and the control ones respectively). Also there were significant (P< 0.05) decreases in the mean values of each of GOT, GPT, ACP and ALP either during preservation at refrigeration conditions or at incubation temperature. Conception rate, Kindling rate and litter size values were significantly (P <0.05) better in rabbit does inseminated artificially by using diluted semen supplemented with 50 μl/ ml aqueous extracts of Korean red ginseng@ than those inseminated using extended semen free- Korean red ginseng extract. In conclusion, addition of Korean red ginseng@ on diluted rabbit semen might improve the semen quality during preservation at different temperatures and fertility traits of rabbits does inseminated artificially. It was found that the ideal level was the addition of Korean red ginseng aqueous extracts with 50 μl /1 r diluted semen.
Key words: Korean red ginseng@, semen preservation, enzymes, A.I fertility.
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| Publication year |
2009
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| Pages |
280-295
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| Availability location |
معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى- الجيزة
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| Availability number |
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| Organization Name |
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| City |
اسيوط
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| serial title |
Assiut Veterinary Medical Journal
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| Author(s) from ARC |
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Enzymes.
Fertility.
Semen preservation.
Spermatozoa.
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| Proposed Agrovoc |
A.I;Korean red ginseng@;sperm motility;
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| Publication Type |
Journal
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