Nutritional evaluation of a fungal-yeast probiotic fortified with natural antioxidants on performance of sheep. 3- impact on body temperature, internal antioxidants, hematoligical and histopathological changes of local sheep raised under different climatic conditions

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Abstract: This study was carried out on two breeds of local sheep to evaluate the nutritional impact of feeding different supplements of the bio-additive EMMH on body temperature, body weight gain, blood picture, blood antioxidants and histopathological statues of liver and kidney tissues. The tested EMMH is a bio-natural feed additive made of T reesei. A. oryzae and active dry yeast S. cerevisiae in co-culture fortified with mutli-herbal antioxidants. Two feeding experiments were carried out on 32 heads of male Rahmany lambs at El-Serw Station (North Western Egypt) for 168 days and on 24 heads of male Farafra lambs at Mallawy Station (Upper Egypt) for 126 days. Lambs of both breeds were of three months old with an initial average weight of 18.04±0.53 kg and 18.53 ±11 kg, respectively. Lambs of each flock were distributed by weight into four similar groups, where each group was individually fed one of four diets supplemented with EMMH at 0, 0.3, 0.4 and 0.5% of the concentrate feed mixture (CFM). Un supplemented or EMMH supplemented CFM were offered at 2.5 % of body weight + 1 % of body weight I3erseem hay for Rahmany and 3 % of body weight CFM for Farafra lambs, while chopped rice straw was offered adlib to both flocks. Minimum and maximum ambient temperature and relative humidity were recorded. Daily gain and rectal temperature were biweekly recorded while blood samples were individually collected twice during the last month of each feeding experiment. By end of the feeding periods, three representative animals were chosen randomly and slaughtered. Specimen of liver and kidney were collected of each carcass and kept in formalin solution for histopathological examination. The results illustrate that, body temperature of animals in experimental groups were within the normal range with no significant difference among groups. However slight stability of body temperature was noticed in groups fed experimental diets. Average daily gain (ADG) was improved (p<0.01) for both breeds with diets contained EMMH and the effect on weight gain was more pronounced with increasing the supplementation level up to 0.5% of CFM. Meanwhile, Farafra sheep exert better response to EMMH supplementation than Rahmany for ADG. No significant differences were attained among groups for blood Hb, g/dI, PVC %. TLC, µL and leukocytic series. µL. However, Hb and PCV tended to increase and neutrophils to decrease with increasing the EMMH supplementation level in diets of both breeds. Significant effect (p<0.05) was recorded for EMMH on blood erythrocytes content of glutathione, glutathione peroixidase and superxide dismutase for Rahmany and Farafra sheep. The highest values of blood antioxidants were obtained with the highest EMMH supplementation level (0.5%). The microscopic examination of liver and kidney tissues showed infiltration of inflammatory cells, necrosis, malformation of the liver cord and partial fibrosis of liver and kidney tissues of sheep fed control diets, whereas the case was more acute in Farafra. The tissues of both organs were visually improved and became normal for animals fed supplemented diets, particularly those in group fed 0.5% EMMH level. The results concluded that the feed additive EMMH had potential influence on improving weight gain and withstanding the high environmental temperatures with advisable EMMH supplementation at 0.5% level of the traditional concentrates mixture.
Keywords: sheep; growth; probiotic; blood picture; blood antioxidants and histopathological examination.

Publication year

2012

Pages

295-310

Availability location

معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى- الجيزة

Availability number

Organization Name

Animal Production Research Institute (APRI)

serial title

Egyptian Journal Nutrition and Feeds

ISSN