Some physiological and immunological measurements in local breed of chickens after heat stress
Abstract: A total number of 500 two-week old sexed chicks from each strain "Inshas and Matrouh" was used to study the effect of heat stress on heat shock protein 70 expression as well as other physiological and immunological responses. The chicks were divided randomly into five equal groups "100 chicks for each group, 50 males and 50 females". The first group didn't receive any treatment and served as control, the second group was exposed to both early (at 4 weeks of age) and late (at 18 weeks of age) heat stress without anti-stress, the third group received the same treatment as second group with anti-stress, the fourth group was exposed to late heat stress without anti-stress, the last group (fifth) was received the same treatment as the fourth group with anti-stress. The heat stress was for 4 hours / day for 5 consecutive days at 38-39 °C inside the house and the anti-stress was vitamin C, where it was added at level of 3 g (20% concentration)/liter drinking water from 1 day before heat exposure until the end of the treatment. The obtained results could be summarized as following:
The Sodium Dodecyl Sulphate - Polyacrylamide gel electrophoresis (SDSPAGE) of proteins in liver samples showed that, exposure to heat stress significantly increased the expression of heat shock protein 70 (HSP70), furthermore, the expression of HSP70 was higher in the treatment with early+ late than the treatment with late heat stress only. Body temperature (°C) in treated chicks was higher significantly (P< 0.05) than control and females were higher significantly (P<0.05) than males by (0.5 °C). Heat exposure increased respiration rate (RR) significantly (P< 0.05) in treated chickens compared with control and in Inshas strain than Matrouh strain. In general, no significant differences were found between two sexes for respiration rate. Heat treatment caused a reduction in hemoglobin (Hb) concentration and hematocrite (Ht %). The heat treatment caused decline in both Plasma Total Protein and Globulin concentration, and elevation in the Albumin concentration, but these differences were not significant.
The effects of strain or sex or their interaction (strain. sex) on these traits were not significant. Late heat exposure with vitamin C increased Albumin concentration significantly (P< 0.05) than other treatments. Heat stress caused an elevation in the Glucose concentration, but this raised was not significant, no differences were found between strains or sexes in the glucose concentration.
Treatment with heat stress, either with anti-stress or without anti-stress had no effect on antibody (Ab's) production (humoral immune response) against sheep red blood cells (SRBC' s). Also no significant differences were found between strains or sexes. With regard to Ab's productions against Newcastle disease (ND), the treatment with heat stress decreased significantly (P<0.05) the humoral immune response against Newcastle disease (ND) compared with control, no significant differences were found between strains or sexes, also, heat treatment caused a decline in the cell mediated immunity, via Phytohemagglutinin (PHA) injection, but this decline was not significant.
Publication year 2006
Pages PP.189
Availability location مكتبة معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى- الجيزة
Availability number 980
Organization Name
Country Egypt
Author(s) from ARC
Agris Categories Animal physiology - Nutrition
AGROVOC
TERMS		 Ascorbic acid. Females. Haemoglobin. Heat shock proteins. Heat stress. Immune response. Immunity. Males. Respiration rate.
Proposed Agrovoc hematocrite;Albumin concentration;
Publication Type Master Thesis
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