|
|
Fertilizing ability of diluted rabbit semen added with bee pollen aqueous extract
|
|
Abstract: A total number of 221 New-Zealand White rabbits (45 sexually mature bucks aged 9 months, and 176 non-parous does aged 6 months) was used in 3 experiments. In experiment 1, semen was collected artificially, pooled and diluted with sodium chloride, diluents (NaCl, 0.9%). Aqueous extracts of Bee-Pollen were added to the diluted semen at levels 0, 50, 100, or 150 µl/ ml, then the diluted semen was stored at refrigeration temperature (4-6°C) for up to 3 days or incubated at 37°C for up to 4 hours. Percentages of sperm motility; dead & abnormal spelmatozoa and acrosomal damages were recorded at the different stages of the preservation. Experiment 2 was designed to evaluate the effect of Bee-Pollen aqueous ; extract on semen contamination with some bacteria present in diluted rabbit semen. In experiment 3, rabbit does were aI1ificially inseminated using either the diluted semen free ,(control) or added with 100 μl Bee-Pollen extract/l ml diluted semen (the level which showed the best effect on semen quality in experiment (l) to estimate the fertility traits.
It was shown that, supplementing Bee-Pollen extract to the diluted rabbit semen significantly (P<0.05) improved the sperm motility, while dead spermatozoa, sperm abnormalities and acrosomal damage percentages were significantly (P<0.05) decreased, uring storage at refrigeration temperature (4-6°C) for up to 3 days or incubation at 37°C for up to 4 hours. Semen quality and storageability (%) were arranged discendingly as obtained by added diluted semen with 150, 100, 50 µl Bee-Pollen extract/ ml than control. Semen quality decreased significantly (P<0.05) with progression of time at different preservation temperatures. Aqueous extracts of Bee-Pollen at 150 and 100 μl/ml showed a better inhibitory effect on the microbial count compared with 50 and 100 µl Bee-Pollen/ml diluted semen.
Key Words: Rabbits, Bee-Pollen, semen quality, preservation, microbes count, fertility.
|
| Publication year |
2013
|
| Pages |
683-694
|
| Availability location |
معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى - الجيزة
|
| Availability number |
|
| Organization Name |
|
| City |
الاسكندرية
|
| serial title |
Egyptian Poultry Science Journal
|
| ISSN |
1110-5623
|
| Author(s) from ARC |
|
| Agris Categories |
Animal feeding
|
AGROVOC
TERMS |
Fertility.
Preservation.
Rabbits.
|
| Proposed Agrovoc |
microbes count;semen quality;Bee-Pollen;acrosomal damages;
|
| Publication Type |
Journal
|