Freezability and DNA integrity of dromedary camel spermatozoa in semen collected by artificial vagina and electro-ejaculator

Abstract: Two methods of semen collection from dromedary camel bulls, including electro-ejaculation (EE) versus conventional artificial vagina (AV) were compared in term of their effects on reaction time, physical semen characteristics, sperm biometry, alkaline comet assay of spermatozoa, sperm freezability and concentration of testosterone, some minerals and biochemicals as well as activity of some enzymes in blood serum of camel bulls.
Results showed that using EE significantly increased reaction time, total sperm output per ejaculate, head length and tail width of spermatozoa, total percentage of sperm head showing comet and serum asprtate transaminase (AST) activity, while significantly decreased sperm cell concentration, serum cholesterol, magnesium, zinc, inorganic phosphorus and testosterone concentrations. However, ejaculate volume, percentages of total motility, forward motility, dead, abnormality and acrosome damage of spermatozoa, head width, tai/length, and total length of spermatozoa, grades of comet assay, sperm freezability. concentration of total proteins and theirfraction, serum Na, Ca and K concentrations and activity of alanine transaminase (ALT) and alkaline phosphatase (ALP) were not affected by collection method Electro-ejaculation is considered as a suitable and repeatable technique for semen collection from dromedary camel bulls when semen collection by conventional artificial vagina is impossible.
Keywords: Dromedary camel, semen collection method, freezability, testosterone
Publication year 2014
Pages 1-11
Availability location معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى - الجيزة
Availability number
Organization Name
serial title Egyptian Journal of Animal Production
ISSN 0302-4520
Author(s) from ARC
Agris Categories Animal physiology - Reproduction
AGROVOC
TERMS
Testosterone.
Proposed Agrovoc freezability;semen collection method;Dromedary camel;electro-ejaculation;conventional artificial vagina;reaction time;physical semen characteristics;sperm biometry;
Publication Type Journal