Semen cryopreservation for two local chicken strains as a tool for conservation of Egyptian local genetic resources
Abstract: Semen cryopreservation is the most practical method for the long term storage of poultry genetic resources. The objective was to compare two chicken strains (El-Salam and Dokki-4) for cryopreservation of sperm, two extenders (Tris-egg yolk and Egg yolk citrate) and two cryoprotectants (glycerol and dimethyl-acetamide) with three levels (3, 7 and 10%). At the beginning of the trial all birds were 42 weeks of age. Semen was collected twice a week from twenty cocks from each strain and 150 hens (75 per each) from the same strains were used for fertility trails. Semen was collected, diluted (1: 4v/v) and analyzed for motility, livability and abnormality at three times (post dilution, equilibration and frozen-thawed)
Results declared that strains had no significant differences for post diluted, post equilibrated and post frozen-thawed traits. Tris egg yolk extender had higher significantly effect on post frozen-thawed motility (29.93±0.53) and livability (31.20±0.52) of spermatozoa than egg yolk citrate extender. The best significant cryoprotectants were 7% Glycerol and 3% DMA for post frozen-thawed motility and livability of spermatozoa for both strains.
Tris egg yolk extender had higher significant recovery rate for post frozen-thawed motility and livability. Among cryoprotectant treatments, 7% glycerol followed by 3% DMA achieved significantly highest response in both post frozen-thawed motility recovery and livability for both strain .
The fertility rates obtained with frozen and thawed spermatozoa with 3%DMA and 7% glycerol yielded fertility percentage 28.42 and 37.76% for El-Salam strain, respectively. The hatchability rates were 88 and 91.43 % for 3%DMA and 7% glycerol in El-Salam strain, respectively. While, Dokki-4 gave 0 % for fertility and hatchability.
Key Words: Chicken strains, cryoprotectants, fertility, hatchability, cryopreservation.
Publication year 2014
Pages 607-618
Availability location معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى- الجيزة
Availability number
Organization Name
City الاسكندرية
serial title Egyptian Poultry Science Journal
ISSN 1110-5623
Author(s) from ARC
External authors (outside ARC)
    محمد احمد السيد قسم الموارد الوراثية - البنك القومى للجينات
Agris Categories Animal husbandry
AGROVOC
TERMS		 Biological preservation. Cryoprotectants. Egg hatchability. Fertility. Freezing.
Proposed Agrovoc Chicken strains;
Publication Type Journal
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