Impact of replacing egg yolk with lecithin on sperm characteristics, bacterial count and fertilizing ability of cryopreserved buffalo semen

Abstract: Soy bean lecithin has been used to replace egg yolk in the semen extender. The aim of this study was to evaluate the effect of replacing egg yolk by soy-lecithin in tris extender on freezability and fertilizing capacity of buffalo semen. Semen from five buffalo bulls was frozen in tris extender containing 0.5, 1.0 and 2% soy-lecithin (treatments) or 15% egg yolk (control). Percentage of motility, livability and intact acrosome spermatozoa and also bacterial count was assessed in diluted, post-equilibrated and post-thawed semen. Results revealed insignificant differences in percentage of sperm motility in diluted semen. However, percentages of sperm motility, livability and intact acrosome spermatozoa increased (P<0.05) and bacterial count decreased (P<0.05) in post-equilibrated and post-thawed semen extended with 0.5% soy-lecithin. These results reflected in the highest conception rate (66.7%) of buffalo cows inseminated with semen containing 05% soy-lecithin compared with 53.3% in semen containing egg yolk (control)
In conclusion, egg yolk could be replaced by soy-lecithin in tris-based extender at a level of 0.5% to increase fertilizing capacity of cryopreserved buffalo semen.
Keywords: Buffalo, frozen semen, sperm, bacterial count, fertility.
Publication year 2014
Pages 353-364
Availability location معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى- الجيزة
Availability number
Organization Name
City المنصورة
serial title Journal Animal and Poultry Production, Mansoura University
ISSN 2090-3642
Author(s) from ARC
Agris Categories Animal physiology - Reproduction
AGROVOC
TERMS
Bacteria. Cell counting. Egg yolk. Fertility. Semen. Water buffaloes.
Proposed Agrovoc frozen semen;Soy bean lecithin;semen extender;
Publication Type Journal