Effect of sperm selection by percoll and swim up techniques on the sex ratio of rabbit offspring
Abstract: This study was conducted to evaluate rabbit the semen characterization before freezing and after thawing. Also, to detelmine the sex ratio of rabbit offspring's after artificial insemination (A1) using semen selected by Percoll or swim up method. Semen was collected from New Zealand white rabbit bucks (n=9) and 45 rabbit does in three groups (15 in each) were inseminated with frozen semen (control, G1), semen selected by 67.5 per cent Percoll (G2) or swim up (G3) methods, respectively.
Semen was processed in tris-buffer extender at a rate of 1:4 Results showed that percentage of progressive motility, livability and intact acrosome spermatozoa decreased (P<0.05) post-dilution, post-equilibration and post-thawing. Using semen selected by Percoll in does A1 resulted in more females (65.8 %) and less males (34.2 %). Using semen selected by swim up method in AI produced more males (75 %) and less females (25 %). Control does produced sex ratio of 51.2 per cent males and 48.8 per cent females. In conclusion, present results showed that, regardless sperm characteristics in post-thawed rabbit semen and fertility results, semen selected by Percoll density at 67.5 per cent and 10 min centrifugation or swim-up can be used for determining the sex ratio of rabbits. This may control the desire to male or female production prior to conception.
KEY WORDS Rabbit, Semen, Percoll, Swim up, Artificial insemination, Sex ratio
Publication year 2014
Availability location معهد بحوث الانتاج الحيواني- شارع نادي الصيد دقي- جيزة
Availability number
Organization Name
serial title The Ajas THE ASIAN JOURNAL OF ANIMAL SCIENCE
ISSN 0976-8963
Author(s) from ARC
Agris Categories Animal physiology - Reproduction
AGROVOC
TERMS		 Artificial insemination. Rabbits. Semen. Sex ratio.
Proposed Agrovoc Percoll;
Publication Type Journal
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