The use of polymerase chain reaction (PCR) technique for the rapid detection and identification of mycoplasma bovis.

Abstract: Current methodologies for detecting and identifying Mycoplasma bovis are time consuming and difficult. In the present work, cultural procedure and polymerase chain reaction (PCR) technique were compared. PCR assay was superior to cultivation method (30.55% and 26.55 respectively).
DNA amplification using the PCR technique was evaluated for rapid identification of Mycoplasma bovis. Two oligonucleotide primers of 24 and 25 bases in length were constructed (based on rRNA genes). The amplification reaction produced a single product of 360 bp in size which was readily detected by agarose gel electrophoresis and ethidium bromide staining
This technique provides a simple and extremely sensitive method for the detection of M. bovis in milk and respiratory tract of infected cattle.
Publication year 2001
Availability location مكتبة معهد بحوث صحة الحيوان 7ش نادى الصيد- الدقى-جيزة0
Availability number
Organization Name
City القاهرة
serial title مؤتمر الجمعية المصرية البيطرية
Department Mycoplasma
Author(s) from ARC
Agris Categories Animal diseasesVeterinary science and hygiene - General aspects
AGROVOC
TERMS		 Cattle. DNA. Infection. Mycoplasma bovis. PCR. RNA.
Publication Type Conference/Workshop
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