EVALUATION OF LOCALLY PREPARED ELISA KITS FORDETECTION AND DIFFERENTIATION BETWEEN MYCOPLASMA GALLISEPTICUM AND MYCOPLASMA SYNOVIAE SPECIFIC ANTIBODIESIN CHICKEN SERUM

Abstract: Indirect enzyme-linked immunosorbent assay (ELISA) was developed for detection of Mycoplasma gallisepticum (MG) and M. synoviae (MS) specific antibodies in chicken sera. Antigen concentrations of 12.5 and 625 ig / well for MG and MS respectively gave the best absorbance values of positive and negative sera. Serum dilution of 1: 100 showed the least non-specific bindings and the large difference between positive and negative sera 1:5000 and 1:10000 dilutions were chosen as the standard conjugate dilutions for MG and MS respectively. The use of phosphate - buffered saline containing 0.05 % Tween 20 for washing and diluting steps resulted in optimal differentiation between homologous and heterogolous sera. Serum chickens 8620 from commercial poultry flocks were tested for MG and MS; serum plate agglutination test was the highest (51.8% and 28% for MG and MS), the ELISA test was 50.2 % and 26.4 % while the HI test was the least (47.3 % and 25% for MG and MS). The results of the present study proved that ELISA was more accurate than HI for confirmation of Mycoplasma gallisepticum and Mycoplasma synoviae infection
Publication year 2001
Organization Name
Country Egypt
City القاهرة
Publisher Name: Volume61 – N0.2
serial title الجمعية المصرية البيطريةالطبية
Department Mycoplasma
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Antibodies. Blood proteins. Chickens. Egypt. Elisa. Mycoplasma gallisepticum. Mycoplasma synoviae.
Publication Type Conference/Workshop
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