Detection of genetic variability among mycoplasma bovis strains by random ampllfied polymorphic dna (RAPD) technique

Abstract: Random amplified polymorphic DNA (RAPD) was used to distinguish among strains of M bovis isolated from cattle and camels. In present study, two ten base oligonucleotide primers were used individually to prime DNA synthesis from genomic DNA of M bovis strains. Results of RAPD-PCR profile of M bovis isolated from cattle and camel showed the presence of variant 1-11 weaker and stronger DNA bands with each isolate. Each isolate was varied from others by the presence or absence of some other bands. The technique can distinguish genetically the different strains of M bovis and provide a valuable tool for epidemiological typing of M bovis isolates
Publication year 2005
Availability location مكتبة معهد بحوث صحة الحيوان 7ش نادى الصيد- الدقى 0
Availability number
Organization Name
City القاهرة
serial title المجلة المصرية للباثولوجيا المقارنة و الباثولوجيا الاكلينيكية
Department Mycoplasma
Author(s) from ARC
Agris Categories Animal husbandry
AGROVOC
TERMS
DNA. Genetic variation. Mycoplasma bovis.
Publication Type Journal