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Detection of genetic variability among mycoplasma bovis strains by random ampllfied polymorphic dna (RAPD) technique
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Abstract: Random amplified polymorphic DNA (RAPD) was used to distinguish among strains of M bovis isolated from cattle and camels. In present study, two ten base oligonucleotide primers were used individually to prime DNA synthesis from genomic DNA of M bovis strains. Results of RAPD-PCR profile of M bovis isolated from cattle and camel showed the presence of variant 1-11 weaker and stronger DNA bands with each isolate. Each isolate was varied from others by the presence or absence of some other bands. The technique can distinguish genetically the different strains of M bovis and provide a valuable tool for epidemiological typing of M bovis isolates
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Publication year |
2005
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Availability location |
مكتبة معهد بحوث صحة الحيوان 7ش نادى الصيد- الدقى 0
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Availability number |
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Organization Name |
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City |
القاهرة
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serial title |
المجلة المصرية للباثولوجيا المقارنة و الباثولوجيا الاكلينيكية
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Department |
Mycoplasma
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Author(s) from ARC |
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Agris Categories |
Animal husbandry
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AGROVOC TERMS |
DNA.
Genetic variation.
Mycoplasma bovis.
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Publication Type |
Journal
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