Preparation of mycoplasma gallisepticum subunit vaccine and its evaluation in chickens
Abstract: A 64 kilodalton (KD) lipoprotein was isolated from M ycopi asnia gall isepticum membrane and used For preparation of subunit vaccine. Evalua tion of the prepared vaccine against colonization of M. gallisepticum (MG) in chicken trachea was studied. Immunoblot of 64 KD protein of M. gal liseplicuin separated by high performance liquid chromatography (HPLC) and Triton X -114 ex traction, against anti- MG chicken serum proved that it is highly immunogenic protein. Sixty one- day old mycoplasma free Hubbard chicks were used. The chicks were divided into 5 groups each of tweleve chicks. Three groups were given 0.25, 0.5 and 0.75 mg of MG vaccine then challenged one month later with 15000 CFU/ 0.1 ml /bird/ iiitratracheally. The remaining two groups were kept as negative and positive controls. Chickens
vaccinated with subunit vaccine (0.5 and 0.75 mg/ bird) resulted in 100% protection against tracheal M. gallisepilcuin colonization . Birds of the group vaccinated with 0.25 mg / bird (group No. I )were positive for mycoplasrna isolation up to 4 weeks post-challenge. Vaccinated groups No (2 and 3) were positive by serum plate agglutina tion test and ELISA along the duration of experi ment. While chickens of group No (1) that vacci nated with 0.25 mg / bird gave suspected results by SPA and ELISA in the first week post- challenge, then gave positive results.
Vaccination of the chickens with M. gallisepticwn subunit vaccine resulted in high antibody re sponse to 64 KD protein at 2 weeks after the booster dose.
Publication year 2002
Availability location مكتبة معهد بحوث صحة الحيوان 7 ش نادى الصيد- الدقى0
Availability number
Organization Name
City القاهرة
serial title المجلة الطبية البيطرية المصرية
Department Mycoplasma
Author(s) from ARC
Agris Categories Veterinary science and hygiene - General aspects
AGROVOC
TERMS		 Chickens. Mycoplasma gallisepticum. Vaccines.
Publication Type Journal
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