Detection of pathogenic mycoplasmas (M. gellisep ticum and m.synoviae)in samples from chickens and turkeys by polymerase chain reaction (pcr) and identificltion by arbitrarily primed pcr (AP-PCR).

Abstract: The polymerase chain reaction (PCR) with primers complementary to the 16s rRNA genes was used to detect avian mycoplasmas. Mycoplasma gallisepticum and M. Synoviaae field and reference strains were successfully amplified by the oligonucleotide primers choiced and gave a characteristic fragment of 369 bp. However, M. puiorum, M. gallinaceum, M.iowae and M. gallinarum were not amplified by these primers.
The arbitrarily primed PCR (AP-PCR) was used demonstrate heterogeneity among strains and isolates of M. gallisepticumi and M. synoviae. Seventeen base oligonucleotide primers were used individually to prime the genomic DNA of M. gallisepticum (MG) and M. synoviae (MS) strains. The MG and MS strains or isolates were identified according to the banding patterns of their DNA. The differences were characteristic for specific strains. Therefore, the PCR method is a useful tool for the detection of pathogenic mycoplasmas (M. gallisepticum and M. synoviae) in samples from chickens and turkeys, while, the AP-PCR fingerprinting is capable of distinguishing the genetic differences among the pathogenic and vaccinal strains of Mycoplasma gallisepticum, and also, between M. gallisepticum and M. synoviae strains.
Publication year 2000
Availability location مكتبة معهد بحوث صحة الحيوان 7 ش نادى الصيد- الدقى0
Availability number
Organization Name
City القاهرة
serial title المجلة المصرية للبحوث الزراعية
Department Mycoplasma
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Mycoplasma gallisepticum. Mycoplasma synoviae. PCR.
Publication Type Journal
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