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Detection of genetic variability among mycoplasma bovis strains by random amplifiedpolymorphic DNA (RAPD) technique
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Abstract: Random amplified polymorphic DNA (RAPD) was used to distinguish among strains of M bovis isolated from cattle and camels. In
present study, two ten base oligonucleotide primers were used individually to prime DNA synthesis from genomic DNA of M bovis strains. Results ofRAPD-PCR profile of M bovis isolated from cattle and camel showed the presence of varient 1-11 weaker and stronger DNA bands with each isolate. Each isolate was varied from others by the presence or absence of some other bands . The technique can distinguishe genetically the different strains of M bovis and provide a valuable tool for epidemiological typing of M bovis isolates.
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| Publication year |
2005
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| Availability location |
مكتبة معهد بحوث صحة الحيوان 7 ش نادى الصيد- الدقى0
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| Availability number |
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| Organization Name |
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| City |
القاهرة
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| serial title |
المجلة المصرية للباثولوجيا المقارنة و الباثولوجيا الاكلينيكية
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| Department |
Mycoplasma
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Genetic variation.
Mycoplasma bovis.
RAPD.
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| Publication Type |
Journal
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