Characterization and immunogenicity of outer membrane proteins (oMPS) of Yersinia enterocolitica isolated from sheep and goats

Abstract: Yersinia enterocolitica serobiovar O: 2/5, O: 9/2 and O;3/5 were isolated from faecal specimens of 11 sheep and serobiovar O:2/5 and O: 3/5 were isolated from faecal spcimens of 3 goat> Infected animals had diarrhoea and were in poor health condition > Thirteen Yersinia enterocolitica, out of 14 isolates (92.8%) were positive for calcium dependency and autoagglutination, 1o isolates (71,4% ) were positive for crystal violet binding and 8 isolates (57.1% ) for guinea pigs produced conjunctivitis .
Yersinia outer membrane proteins were extracted from different serovare . These Omps PAGE and all of them gave seven protein bands with molecular weight ranging from 23 to 62 KDa
Preparation of OMPs successfully protected mice (100% ) .After immunization with OMP , mice showed an increas in antibody titer to OMP preparation by ELISA and microagglutination test .
These results indicated that the OMP preparation elicit a specitic humoral antibody response in immunized animals .
Publication year 2001
Pages 101-91
Availability location مكتبة معهد بحوث صحة الحيوان
Availability number
Organization Name
City القاهرة
serial title مجلة الجمعية الطبية البيطرية المصرية
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS
Goats. Sheep. Yersinia enterocolitica.
Publication Type Conference/Workshop