Growth and survival of Escherich coli carrying multidrug resistance plamids during preparation and storage of yoghurt with reference to their public health hazard

Abstract: Through an investigation of hovine snbclinicalinastitic milk samples,-ff-raff strains were Isolated and biochemically identified, they were ontypaBle after testing against available niBe serotypes. E. cff//isolates were subjected to antimicrobial susceptibility testing against 13 antimicrobial agents (cefobid, oflosaclllin, claforan, streptomycin, spiramycin, cephradin, closacillin. ampicillin, unasyn, amosyciilln, penicillin G, ciprocin and polymexinE.sulphate). Plasmid profile analysis was carried out to 10 mnltidrng resistance £c»//strains, of which, only fonr isolates were carrying different plasmids with molecular sizes (5.3-7.5!(»)-An £co// strain carrying fonr plasmids was selected to be inoculated into sterile milk (pH 6.6) with initial nose of \.3y• 10' cellsJml after adding the ynghurt starter cultures and incubation at 37±l°C. In the finish product, E.eff/i covnt was S^IO6 and the pH was 5.24. The yoghurt ttllh Its cuntrol were stored at 4±1°C for 7 days- Slow decline in E.eellcawtt. was observed, where the E.coli strains survived till the end of the week and persisted in large nBinbers^.T^lO5 cellsfml) ana resisted the acidity of pH 4.03. Public health hazard of yoghnrt contamination with such A'-iW// strains was discussed. An experiment was carried out to study the probability of plasmid transmission from E.eoU strains to starter organisms. Yoghurt was prepared from two types of starters. One of them lived and stored with the tested £c
Publication year 2002
Pages 65 – 72
Availability location مكتبة معهد بحوث صحة الحيوان
Availability number
Organization Name
serial title مجلة البيئة –العدد 5 – مارس 2002 - من صفحة 65 – 72
Author(s) from ARC
Agris Categories Animal diseases
Publication Type Journal