Electrophretic analysis of four local populations of Nile tilapia وOreochromis niloticus in Egypt
Abstract: Four local populations of Nile tilapia (Oreochromis niloticus) were collected from different locations in Egypt namely, Abbassa, Maryout, Manzala and Aswan. protein banding patterns (SDS-PAGE) for all populations were performed using soluble fraction of muscle extract. Furthermore, isozymes banding patterns for each population was performed using eye extract. Total band numbers detected in each individual population sample were scored, compared, analyzed and assessed for the four-izosyme system ; Esterase (Est), Malate dehydrogenase (Mdh), Alcohol dehydrogenase (Adh), Lactate dehydrogenase (Ldh). The DNA markers were concluded applying RAPD-PCR technique. Two arbitrary random primers were tested. All primers (OPA-05 and B-19) amplified genomic DNA and produced distinct bands. The variation among and between populations were estimated and compared with that of both isozymes and proteins. The data concluded from each genetic criterion was pooled to draw the genetic relations among and between populations. The intra-specific variation for SDS-PAGE was low for both Abbassa and Manzala and high for Maryout and Aswan. Abbassa resembled Manzala and Maryout population showed more polymorphism. The esterase izosyme of Maryout and Manzala populations showed high similarity. The Adh of Manzala and Aswan populations showed high similarity. The Ldh showed inter-specific variation in both Maryout and Manzala, and no variation in both Abbassa and Aswan. The primer OPB-19 displayed high variation in RAPD fragment among four applied populations. Both Abbassa and Manzala populations had the highest value of similarity coefficient, followed by Maryout /Aswan. Also, the results indicated no similarity between Abbassa/Maryout and between Maryout/Manzala.
The results generated from the RAPD, isozyme, and protein analysis were pooled for drawing the genetic relationships among the four examined populations. The results revealed that the highest similarity coefficient value (0.83) was observed between Manzala and Maryout followed by that Abbassa and Aswan (0.79). The similarity coefficient value between Aswan and each of Maryout and Manzala showed the lowest value (0.65 and 0.69), respectively. The phylogenetic tree indicated that Maryout and Manzala form one group that branched to the subgroup of Abbassa, which branched in turn to another subgroup of Aswan population.
Publication year 2002
Pages pp107-129
Availability location المعمل المركزى لبحوث الثروة السمكية
Availability number
Organization Name
Country Egypt
City Cairo
serial title مجلة الجمعية الاكاديمية المصرية لتنمية البيئة
Volume 3 . 1
Department fish health and zoonosis
Author(s) from ARC
Agris Categories Animal genetics and breedingFisheries production
AGROVOC
TERMS		 DNA. Egypt. Electrophoresis. Isoenzymes. Oreochromis niloticus. PCR.
Publication Type Journal
↑